General Cell Based Assays
TRIBIOSCIENCE INC Neuraminidase Activity Fluorometric Assay 100 Tests
The Neuraminidase Activity Fluorometric Assay provides a simple, accurate, and sensitive method for monitoring neuraminidase activity in biological samples. In this assay, neuraminidase cleaves a non-fluorescent substrate to release a fluorescent product, which can be measured at an excitation wavelength of 365 nm and an emission wavelength of 445 nm. Applications - Neuraminidase activity in biological samples. Infuenza virus antigen titration. Key Features - Simplicity - The assay directly detects enzymatic activity without complex enzyme coupling steps. Sensitivity - Even low levels of neuraminidase activity can be detected due to the high fluorescence intensity.
Crystal Chem Inc Mouse Glucose Assay Kit, 96-tests
The Mouse Glucose Assay is used to quantify levels of glucose in mouse serum, plasma, and urine using only a 2 µL sample volume. Highlights: Easy-to-use, results in 5 minutes. Kits use a sample size of only 2 µL. For use with serum, plasma, and urine. Designed for mouse samples. Crystal Chem is a worldwide leading supplier of mouse and rat ELISA kits for research use. Our kits are among the highest quality found in the market, and are used by leading research universities, pharma companies, and government institutions around the world.
Bioassay Systems QuantiChrom™ Urease Assay Kit. For quantitative determination of urease activity and evaluation/screen for urease inhibitors.
For quantitative determination of urease activity and evaluation/screen for urease inhibitors. Key Features: Safe. Non-radioactive assay. Sensitive and accurate. As low as 0.003 U/L urease activity can be quantified. Homogeneous and convenient. "Mix-incubate-measure" type assay. No wash and reagent transfer steps are involved. Robust and amenable to HTS: can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: OD670nm; Samples: Biological, environment etc; Size: 100 tests.
Bioassay Systems EnzyChrom™ Lactose Assay Kit. For quantitative determination of lactose and evaluation of drug effects on lactose metabolism.
For quantitative determination of lactose and evaluation of drug effects on lactose metabolism. Key Features: Sensitive. Use as little as 20 µL samples. Linear detection range in 96-well plate: 17 to 2000 µM lactose for colorimetric assays and 6 to 100 µM for fluorimetric assays. Convenient and high-throughput. Robust and homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: OD570nm, or FL530/585nm. Samples: Biological (milk), food and agriculture etc. Species: All. Procedure: Assay takes 30 min. Kit size: 100 tests. Detection limit: 6 µM.
Eurofins Discoverx cAMP Hunter™ Human PPYR1 Gi Cell-Based Assay Kit (CHO-K1)
The cAMP Hunter™ Human PPYR1 Gi Stable Cell Line Assay (CHO-K1) contains a stable clonal cell line used to measure PPYR1 (GPCR) activity via decreased levels of intracellular cAMP. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eagle Biosciences Inc Ultra Sensitive Colorimetric Assay for Nitric Oxide Synthase
Ultra Sensitive Colorimetric Assay for Nitric Oxide Synthase
Eurofins Discoverx PathHunter eXpress PTGFR Total Internalization Assay, 10pl Kit. (93-0872E3CP0L)
The PathHunter PTGFR Total Internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit uses EFC technology to measure ligand-induced internalization of PTGFR via detection of localization to the early endosome. The included cell line overexpresses ProLink-tagged PTGFR and an EA-tagged early endosome protein. Upon ligand-induced receptor activation, resulting internalization of the PTGFR receptor to the early endosome leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Meso Scale Discovery R-PLEX Human EGFR ASY
The R-PLEX Human EGFR Assay Kit from Meso Scale Diagnostics is a multiplex immunoassay measuring total EGFR levels in human serum plasma and cell culture supernatants This electrochemiluminescence (ECL) assay uses MSD s SULFO-TAG labels and multi-array plate technology for simultaneous detection of multiple protein biomarkers from a single sample It offers high sensitivity broad dynamic range low sample volume and rapid results The kit includes a 96-well plate with capture antibody detection antibody calibrator and assay diluent suitable for biomarker discovery disease research and drug development
Eurofins Discoverx PathHunter eXpress CSF1R/CSF1R Dimerization Assay, 2pl Kit. (93-1061E3CP5M)
The PathHunter CSF1R/CSF1R Dimerization eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting receptor-receptor interaction. This kit assesses ligand (e.g. M-CSF) induced dimerization of CSF1R/CSF1R receptors. This product uses EFC technology. The cell line included overexpresses CSF1R target protein fused to PK alongside CSF1R target protein fused to EA. Upon ligand-induced dimerization of CSF1R/CSF1R, detectable EFC signal is produced. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx cAMP Hunter Teriparatide Bioassay Kit, 10pl. (95-0118Y2-00058)
The cAMP Hunter Teriparatide Bioassay kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay assesses Teriparatide based activation of PTHR GPCR activity via detection of levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type PTHR, a naturally Gs coupled GPCR. Upon activation of PTHR, the increase in levels of intracellular cAMP leads to an increased signal from EFC when the cAMP detection kit is applied. Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates. For detailed product information, User manual and COA, visit www.discoverx.com/bioassay.
Eurofins Discoverx PathHunter eXpress GPR123 CHO-K1 β-Arrestin Orphan GPCR Assay, 2-Plate Kit. (93-0417E2ACP1M)
The PathHunter GPR123 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand based activation of GPR123 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GPR123 and EA-tagged β-Arrestin-2. Activation of GPR123 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. This is an orphan receptor without a known ligand. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
American Research Products Inc ELF5 Transcription Factor Activity Assay
ELF5 Transcription Factor Activity Assay
Meso Scale Discovery MAP Kinase WCL20ate
The MAP Kinase Whole Cell Lysate Kit from Meso Scale Discovery provides reagents to detect total and phosphorylated ERK1/2 JNK1/2 and p38 MAP kinases in cell lysates The kit contains a 96-well plate coated with capture antibodies detection antibodies and buffers needed to perform the assay The electrochemiluminescent detection allows for high sensitivity and a wide dynamic range This kit allows for the quantitative analysis of MAP kinase activation in cells With a single 96-well plate up to 40 samples can be analyzed in triplicate
Meso Scale Discovery R-PLEX Human S100A8/MRP8 ASY
The R-PLEX Human S100A8 (MRP8) Assay Kit from Meso Scale Diagnostics is a multiplex immunoassay that measures S100A8 protein levels in human serum plasma and cell culture supernatants The assay uses electrochemiluminescence (ECL) detection technology and provides high sensitivity broad dynamic range and requires low sample volume The kit includes a 96-well plate coated with capture antibody detection antibody read buffer wash buffer sample diluent and calibrators and controls The assay has a typical sensitivity of 0 3 pg/mL and an upper limit of quantification of 10 000 pg/mL It is for research use only
Bioassay Systems EnzyChrom™ Adipolysis Assay Kit. For quantitative assay of adipolysis and evaluation of drug effects on adipolysis.
For quantitative assay of adipolysis and evaluation of drug effects on adipolysis. Key Features: Sensitive and accurate. Use as little as 10 µL samples. Linear detection range in 96-well plate: 0.92 to 100 µg/mL (10 to 1000 µM) glycerol for colorimetric assays and 0.2 to 5 µg/mL for fluorimetric assays. Rapid and convenient.The procedure involves addition of a single working reagent and incubation for 20 min at room temperature. Robust and amenable to HTS assays. Potential interference by testing drugs is greatly reduced at 570nm. Compatible with culture media containing phenol red. Assays can be performed in 96 or 384-well plates. Method: OD570nm, or FL530/585nm. Samples: Cell culture media. Species: All. Procedure: Assay takes 20 min. Kit size: 200 tests. Detection limit: 0.92 µg/mL.