General Cell Based Assays
Meso Scale Discovery R-PLEX Rat MIP-3 ASY
The R-PLEX Rat MIP-3alpha Assay Kit from Meso Scale Discovery is a multiplex immunoassay for measuring rat MIP-3alpha in various biological fluids It uses MSD s electrochemiluminescent detection for high sensitivity and a wide dynamic range with low sample volume An MSD instrument like QuickPlex is needed to read the plate The kit includes a 96-well plate with capture and detection antibodies plus calibrators and controls Detection limits are 1 52 pg/mL to 1500 pg/mL for rat MIP-3alpha
Eurofins Discoverx PathHunter eXpress MRGPRD CHO-K1 β-Arrestin GPCR Assay, 10-Plate Kit. (93-0926E2CP2L)
The PathHunter MRGPRD eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. β-alanine) based activation of MRGPRD GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged MRGPRD and EA-tagged β-Arrestin-2. Activation of MRGPRD stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress EDG1 CHO-K1 β-Arrestin GPCR Assay, 10-Plate Kit. (93-0207E2CP4L)
The PathHunter EDG1 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. S1P) based activation of EDG1 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged EDG1 and EA-tagged β-Arrestin-2. Activation of EDG1 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Genscript Corporation ToxinSensor Single Tests Kit
GenScript ToxinSensor Single Tests Kit with Standard is designed to be a qualitative In Vitro end-point endotoxin test for human and animal parenteral drugs biological products and medical devices Tachypleus Amebocyte Lysate (TAL) as supplied is to be reconstituted with sample or control directly After incubation for 1 hour and in the presence of endotoxin gelation occurs in the absence of endotoxin gelation does not occur GenScript supply a series of ToxinSensorTM Single Tests Kits with Standard with different sensitivity (0 03 EU/ml 0 06 EU/ml 0 125 EU/ml and 0 25 EU/ml)
Eurofins Discoverx cAMP Hunter eXpress OPRD1 CHO-K1 GPCR Assay, 10-Plate Kit. (95-0108E2CP2L)
The cAMP Hunter OPRD1 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. DADLE, DPDPE) based activation of OPRD1 GPCR activity via detection of decreasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type OPRD1, a naturally Gi/Go coupled GPCR. Upon activation of OPRD1, the decrease in levels of intracellular cAMP leads to a decreased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx cAMP Hunter eXpress SSTR4 CHO-K1 GPCR Assay, 10-Plate Kit. (95-0059E2CP2L)
The cAMP Hunter SSTR4 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. somatostatin 28) based activation of SSTR4 GPCR activity via detection of decreasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type SSTR4, a naturally Gi/Go coupled GPCR. Upon activation of SSTR4, the decrease in levels of intracellular cAMP leads to a decreased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter Growth Hormone Bioassay Kit, 2pl. (93-0756Y3-00023)
The PathHunter Growth Hormone Bioassay kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay assesses human growth hormone-induced activation of GHR cytokine receptors via detection of SH2 domain protein recruitment. The included cell line overexpresses ProLink-tagged GHR target protein and EA-tagged SH2 domain protein. Upon activation of the receptor by its ligand or an agonist antibody, recruitment of SH2 domain protein to the phosphorylated receptor tail leads to production of EFC signal. Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates. For detailed product information, User manual and COA, visit www.discoverx.com/bioassay.
Aat Bioquest Cell Meter™ Mitochondrial Hydroxyl Radical Detection Kit *Red Fluorescence*
The detection of intracellular hydroxyl radical is of central importance to understanding proper cellular redox regulation and the impact of its dysregulation on various pathologies.
Eurofins Discoverx PathHunter eXpress CALCRL-RAMP2 (AM1) CHO-K1 β-Arrestin GPCR Assay, 10pl Kit. (93-0270E2CP0L)
The PathHunter CALCRL-RAMP2 (AM1) eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. adrenomedullin) based activation of CALCRL GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged CALCRL and EA-tagged β-Arrestin-2, along with untagged modulating protein RAMP2. Activation of CALCRL stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
AURORA BIOLABS LLC Kras Q61K Nucleotide Exchange Assay Kit,100 rxn
KRAS is a small GTPase in the RAS protein family that plays a critical role in regulating cell signaling pathways involved in proliferation, differentiation, and survival. KRAS cycles between an inactive GDP-bound state and an active GTP-bound state, and activation occurs through nucleotide exchange from GDP to GTP. Mutations such as KRAS Q61K are frequently associated with human cancers and contribute to abnormal signaling and uncontrolled cell growth. The KRAS Q61K Nucleotide Exchange Assay is a TR-FRET–based assay designed to quantitatively monitor nucleotide exchange activity of KRAS Q61K. The assay detects changes in fluorescence resonance energy transfer generated during nucleotide exchange, enabling measurement of KRAS activation and evaluation of compounds that modulate KRAS signaling for cancer research and drug discovery applications.
Eurofins Discoverx PathHunter eXpress CRHR1 CHO-K1 β-Arrestin GPCR Assay, 10-Plate Kit. (93-0225E2CP0L)
The PathHunter CRHR1 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. CRH, sauvagine) based activation of CRHR1 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged CRHR1 and EA-tagged β-Arrestin-2. Activation of CRHR1 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Meso Scale Discovery U-PLEX Mouse KC/GRO ASY
The U-PLEX Mouse KC Assay K152VWK from Meso Scale Discovery is a multiplex immunoassay kit for measuring cytokines and chemokines in mouse samples It uses electrochemiluminescence detection to measure up to 10 analytes per well from a single sample The kit includes a 96-well plate detection antibodies calibrator solutions and buffers It requires 25 L of sample per well and can quantify IL-1 IL-2 IL-4 IL-5 IL-6 IL-10 IL-12p70 IFN- TNF- and KC/GRO in various biological fluids
Eurofins Discoverx PathHunter eXpress SSTR1 U2OS β-Arrestin GPCR Assay, 2-Plate Kit. (93-0307E3CP5M)
The PathHunter SSTR1 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. somatostatin) based activation of SSTR1 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged SSTR1 and EA-tagged β-Arrestin-2. Activation of SSTR1 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Meso Scale Discovery R-PLEX Human P-Cadherin ASY
The R-Plex Human P-Cadherin Assay is a multiplex immunoassay kit that enables the simultaneous quantitative measurement of P-Cadherin in human serum plasma and cell culture supernatants It utilizes Meso Scale Discovery electrochemiluminescent detection technology and provides high sensitivity broad dynamic range and intra- and inter-assay precision This assay requires an MSD instrument and provides components for one 96-well plate including one standard curve comprised of eight points ranging from 3 2 pg/mL to 10 000 pg/mL P-Cadherin